|
KMID : 1161520120160050376
|
|
Animal Cells and Systems
2012 Volume.16 No. 5 p.376 ~ p.384
|
|
|
The role of ginseng total saponin in transient receptor potential melastatin type 7 channels
|
|
Kim Byung-Joo
|
|
|
Abstract
|
|
|
|
Although ginsenosides have a variety of physiologic or pharmacologic functions in various regions, there are only a few reports on the effects of transient receptor potential melastatin 7 (TRPM7) channels. Here, we showed evidence suggesting that TRPM7 channels play an important role in ginseng total saponin (GTS)-mediated cellular injury. The combination techniques of electrophysiology, pharmacological analysis, small interfering RNA (siRNA) method and cell death assays were used. GTS depolarized the resting membrane potentials and decreased the amplitude of pacemaker potentials in cultured interstitial cells of Cajal (ICCs) in gastrointestinal (GI) tract. The TRPM7-like currents in single ICCs and the overexpressing TRPM7 in HEK293 cells were inhibited by GTS. However, GTS had no effect on Ca2 +-activated Cl? conductance. GTS inhibited the survival of human gastric (AGS) and breast (MCF-7) adenocarcinoma cells. Also, GTS inhibited the TRPM7-like currents in AGS and MCF-7 cells. The GTS-mediated cytotoxicity was inhibited by TRPM7-specific siRNA. In addition, we showed that overexpression of TRPM7 channels in HEK293 cells was inhibited by GTS. Thus, TRPM7 channels are involved in GTS-mediated cell death in AGS and MCF-7 cells, and these channels may represent a novel target for physiological disorders where GTS plays an important role.
|
|
|
KEYWORD
|
|
|
transient receptor potential melastatin 7, TRPM7, ginseng total saponin, AGS cells, MCF-7 cells, apoptosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|